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The direction of synthesis for a new mRNA molecule is _______ from a _______ template strand.


A) 5´ to 3´; 5´ to 3´
B) 5´ to 3´; 3´ to 5´
C) 3´ to 5´; 5´ to 3´
D) 3´ to 5´; 3´ to 5´
E) 5´ to 5´; 3´ to 3´

F) A) and B)
G) A) and C)

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Single-stranded RNA replicates by


A) making an RNA strand that is complementary to the original RNA strand.
B) using an adapter molecule.
C) the process of transcription.
D) the process of translation.
E) the process of reverse transcription.

F) A) and D)
G) C) and D)

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The addition of phosphate groups to polypeptide chains following translation is called _______.

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Poly uracil codes for


A) three different amino acids.
B) poly tryptophan.
C) mRNA.
D) a fatty acid.
E) phenylalanine.

F) B) and C)
G) All of the above

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Activating enzymes


A) are sometimes referred to as the second genetic code.
B) link the right tRNAs and amino acids.
C) are also known as aminoacyl-tRNA synthetases.
D) are specific for one amino acid.
E) All of the above

F) A) and B)
G) C) and D)

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The one-gene, one-enzyme hypothesis resulted from the work of _______.

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Beadle and Tatum

During translation initiation, the first site occupied by a charged tRNA is the


A) A site.
B) B site.
C) large subunit.
D) T site.
E) P site.

F) B) and E)
G) A) and B)

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If codons were read two bases at a time instead of three bases at a time, how many different possible amino acids could be specified?


A) 16
B) 64
C) 8
D) 32
E) 128

F) All of the above
G) D) and E)

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A transcription start signal is called a(n)


A) initiation codon.
B) promoter.
C) origin.
D) operator.
E) nonsense codon.

F) B) and E)
G) B) and D)

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B

Processing of pre-mRNA in eukaryotes involves


A) splicing out exons from the primary transcript.
B) adding a poly-A tail to the 5' end.
C) adding a G-cap to the 5' end.
D) scrambling gene sequences.
E) transport of the primary transcript to the cytoplasm.

F) B) and D)
G) A) and B)

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Posttranslational alterations of proteins


A) can affect the activity of an enzyme.
B) may operate in signal transduction.
C) include the addition of polysaccharides to proteins.
D) include the cleavage of signal sequences.
E) All of the above

F) B) and D)
G) A) and B)

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The tail added to pre-mRNA is


A) coded for by DNA.
B) composed of poly T.
C) important for mRNA stability.
D) attached to its 5´ end.
E) All of the above

F) A) and C)
G) B) and D)

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A mutation occurs such that a spliceosome cannot remove one of the introns in a gene. What effect will this have on that gene?


A) It will have no effect; the gene will be transcribed and translated into protein.
B) Transcription will terminate early and the protein will not be made.
C) Transcription will proceed, but translation will stop at the site where the intron remains.
D) Translation will continue, but a nonfunctional protein will be made.
E) Translation will continue and will skip the intron sequence.

F) A) and B)
G) C) and D)

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In eukaryotic cells, proteins that contain covalently attached sugar residues are translated


A) in the nucleus.
B) in the cytoplasm.
C) in mitochondria.
D) on the endoplasmic reticulum.
E) on the Golgi apparatus.

F) A) and D)
G) C) and D)

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A protein that is destined to be excreted from the cell that is making it


A) has a signal sequence that targets it to the plasma membrane.
B) is made solely by ribosomes free in the cytosol.
C) has a signal sequence that stops translation and directs the protein and the ribosome to which it is attached to the endoplasmic reticulum.
D) is targeted to the endoplasmic reticulum as a soluble protein.
E) is targeted directly to lysosomes.

F) A) and D)
G) A) and E)

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Biologists often look for organisms that are easy to grow in the laboratory and that demonstrate the phenomenon they wish to study. These organisms are called _______ organisms.

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Which of the following statements about codons and anticodons is true?


A) The codon bonds covalently with the anticodon.
B) The base sequences are the same.
C) There are 64 codons and 61 anticodons.
D) Activating enzymes link codons and anticodons.
E) At contact, the codon and the anticodon are antiparallel to each other.

F) B) and E)
G) A) and B)

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Which of the following does not occur after eukaryotic mRNA is transcribed?


A) Binding of RNA polymerase to the promoter
B) Capping of the 5´ end
C) Addition of a poly A tail to the 3´ end
D) Splicing out of the introns
E) Transport to the cytosol

F) None of the above
G) D) and E)

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A gene product can be


A) an enzyme.
B) a polypeptide.
C) RNA.
D) microRNA.
E) All of the above

F) A) and B)
G) A) and C)

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Promoters are made of


A) proteins.
B) carbohydrates.
C) lipids.
D) nucleic acids.
E) amino acids.

F) C) and D)
G) B) and C)

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D

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