Question 1

A positive serological test for tuberculosis indicates that the patient

Accepted Answer

A)  has active tuberculosis.
B)  is an asymptomatic carrier of tuberculosis.
C)  has been exposed to tuberculosis.
D)  is immune to tuberculosis.E) A) and B)
F) All of the above

Question 2

The indirect ELISA test is commonly used to diagnose infections caused by all of the following except

Accepted Answer

A)  rubella virus.
B)  HIV.
C)  hepatitis A and C.
D)  Helicobactor.E) C) and D)
F) B) and D)

Question 3

The tuberculin skin test is read

Accepted Answer

A)  within 1 hour.
B)  after 12 hours.
C)  from 12-24 hours.
D)  from 24-48 hours.
E)  from 48-72 hours.F) B) and C)
G) C) and D)

Question 4

You are the supervisor of a new reference microbiology laboratory,which will receive clinical specimens for identification of bacterial pathogens.When choosing the types of media to order for culture and isolation procedures in your laboratory,what question(s) do you need to answer?

Accepted Answer

A)  What type of bacteria will you be culturing?
B)  What are the growth factors required by the bacteria that you will be identifying?
C)  What kind of microbial flora will the patient specimens contain?
D)  All statements are correct.E) A) and D)
F) A) and C)

Question 5

The direct ELISA test is used to diagnose all of the following except

Accepted Answer

A)  hantavirus.
B)  rubella virus.
C)  past exposure to Mycobacterium tuberculosis.
D)  Toxoplasma.E) None of the above
F) All of the above

Question 6

You have a very sore throat with symptoms indicating strep throat.Since you are taking microbiology right now,you decide that you will take a swab from your throat and swab it onto an enrichment medium like a blood agar plate or a brain-heart infusion agar plate.Why is this not a good idea?

Accepted Answer

A)  Due to the fact that you cannot easily see the sampling site,there is a high probability that you will not be able to obtain your own specimen without contaminating the sample with your own normal biota.
B)  You are on a diet now,and you are afraid that the food that you are eating will in some way impact the growth of the bacteria.As a result,you will not get valid results.
C)  You use a mouthwash a couple of times a day,so you think that the antibacterial properties of the mouthwash will inhibit or kill the oral bactera,not giving you valid results.
D)  All of these statements are correct.E) B) and C)
F) A) and D)

Question 7

In precipitation tests,the antigen

Accepted Answer

A)  is a soluble molecule.
B)  is an insoluble molecule.
C)  is a whole cell.
D)  -antibody complex settles to the bottom of the tube.E) B) and C)
F) A) and C)

Question 8

Which of the following is not a genotypic method of identification?

Accepted Answer

A)  rRNA sequencing
B)  pulse-field gel electrophoresis
C)  G + C composition
D)  direct antigen testing
E)  DNA analysis with probesF) A) and E)
G) B) and E)

Question 9

Microarrays,hybridization tests,and ribotyping are increasing in frequency in their use in clinical labs for diagnosis of infectious diseases and the microorganisms causing them.Why is this?

Accepted Answer

A)  They are cheaper than biochemical tests.
B)  They have greater specificity for the organism because they employ specific antibody.
C)  Genomic testing has less error compared to biochemical tests and serological tests.
D)  The tests are more easily performed in a doctor's office or hospital,right there where the specimens are taken.E) C) and D)
F) B) and D)

Question 10

Dr.John Doe,a research microbiologist for a university medical center,has been accused of sending Bacillus anthracis,the causative agent of anthrax,through the mail to the President.You,as a public health official with the CDC,have to determine if it is the same strain as the two strains of B. anthracis found cultured in his lab.Which method of identification would you use to compare these three bacterial samples?

Accepted Answer

A)  serological testing using agglutination
B)  Gram staining
C)  biochemical test identification
D)  pulse-field gel electrophoresisE) None of the above
F) A) and B)

Question 11

The polymerase chain reaction can be used for identification of:

Accepted Answer

A)  antibodies in the patient's serum.
B)  microorganisms.
C)  proteins.
D)  growth characteristics of microorganisms.E) B) and D)
F) B) and C)

Question 12

The patient's vaginal specimen is sent from the walk-in clinic to the microbiology laboratory.There it is cultured on bacteriological media,and then placed into an ambient air incubator set at 22% oxygen and 37 degrees Celsius.The patient is strongly suspected of having gonorrhea.She had sexual contact with a partner recently diagnosed with gonorrhea,and is now displaying the typical symptoms of a vaginal exudate or fluid.Neisseria gonorrhoeae is a microaerophilic gram-negative coccus-shaped bacterium.Seventy-two hours later,the culture has still not grown Neisseria gonorrhoeae so the lab reports the results back as negative.Interpret this result.

Accepted Answer

A)  The clinical personnel took the sample from an incorrect body area.
B)  The culture was placed into the wrong environment for Neisseria gonorrhoeae.
C)  The inoculating loop was too hot after heating,while streaking the specimen onto agar medium,and the bacteria were all killed.
D)  Neisseria gonorrhoeae cannot be grown in bacteriological media and requires genetic analysis for identification.E) A) and C)
F) B) and C)

Question 13

The tuberculin test is an example of an in vitro serological test.

Accepted Answer

A) True 
 B)False

Question 14

The property of a test to detect even small amounts of antibodies or antigens that are test targets is

Accepted Answer

A)  cross-reaction.
B)  agglutination.
C)  precipitation.
D)  specificity.
E)  sensitivity.F) A) and E)
G) A) and B)

Question 15

When antibodies or other substances in serum cross-react with the test reagents,a false positive result can occur.

Accepted Answer

A) True 
 B)False

Question 16

Serological testing can be used to test each of the following except

Accepted Answer

A)  blood serum.
B)  cerebrospinal fluid.
C)  urine.
D)  saliva.
E)  skin.F) A) and C)
G) A) and E)

Question 17

Precipitation tests involve all of the following except

Accepted Answer

A)  they rely on formation of visible clumps for detection.
B)  they include the VDRL test for syphilis.
C)  they are often performed in agar gels.
D)  they can be done in a test tube by carefully adding antiserum over antigen solution.
E)  a cloudy or opaque zone developing where antigen and antibody react.F) A) and B)
G) B) and D)

Question 18

Alkaline phosphatase is an enzyme used in _____ tests.

Accepted Answer

A)  Western blot
B)  ELISA
C)  direct fluorescent antibody
D)  indirect fluorescent antibodyE) A) and D)
F) A) and B)

Question 19

Which of the following is not an agglutination test?

Accepted Answer

A)  counterimmunoelectrophoresis
B)  rapid plasma reagin (RPR) test
C)  Weil-Felix reaction
D)  blood typing
E)  Widal testF) B) and D)
G) C) and E)

Question 20

Which statement about clinical identification tests is true?

Accepted Answer

A)  Not all diseases are identified by clinical tests.
B)  Biochemical tests are quicker than genetic analysis tests.
C)  Serological tests are always based on identifying antibodies in the patient.
D)  Microarray tests are based on electrophoresis methodology.E) B) and D)
F) None of the above

Exam 17: Diagnosing Infections

Precipitation tests involve all of the following except

Correct Answer
verified

Correct Answer
verified

The property of a test to detect even small amounts of antibodies or antigens that are test targets is

Correct Answer
verified

Alkaline phosphatase is an enzyme used in _____ tests.

Correct Answer
verified

You have a very sore throat with symptoms indicating strep throat.Since you are taking microbiology right now,you decide that you will take a swab from your throat and swab it onto an enrichment medium like a blood agar plate or a brain-heart infusion agar plate.Why is this not a good idea?

Correct Answer
verified

Correct Answer
verified

When antibodies or other substances in serum cross-react with the test reagents,a false positive result can occur.

Correct Answer
verified

You are the supervisor of a new reference microbiology laboratory,which will receive clinical specimens for identification of bacterial pathogens.When choosing the types of media to order for culture and isolation procedures in your laboratory,what question(s) do you need to answer?

Correct Answer
verified

Which statement about clinical identification tests is true?

Correct Answer
verified

The indirect ELISA test is commonly used to diagnose infections caused by all of the following except

Correct Answer
verified

A positive serological test for tuberculosis indicates that the patient

Correct Answer
verified

Microarrays,hybridization tests,and ribotyping are increasing in frequency in their use in clinical labs for diagnosis of infectious diseases and the microorganisms causing them.Why is this?

Correct Answer
verified

Which of the following is not an agglutination test?

Correct Answer
verified

Serological testing can be used to test each of the following except

Correct Answer
verified

In precipitation tests,the antigen

Correct Answer
verified

The direct ELISA test is used to diagnose all of the following except

Correct Answer
verified

The tuberculin skin test is read

Correct Answer
verified

Which of the following is not a genotypic method of identification?

Correct Answer
verified

The polymerase chain reaction can be used for identification of:

Correct Answer
verified

The tuberculin test is an example of an in vitro serological test.

Correct Answer
verified

Exam 17: Diagnosing Infections

Precipitation tests involve all of the following except

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

The property of a test to detect even small amounts of antibodies or antigens that are test targets is

Correct AnswerverifiedShow Answer

Alkaline phosphatase is an enzyme used in _____ tests.

Correct AnswerverifiedShow Answer

You have a very sore throat with symptoms indicating strep throat.Since you are taking microbiology right now,you decide that you will take a swab from your throat and swab it onto an enrichment medium like a blood agar plate or a brain-heart infusion agar plate.Why is this not a good idea?

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

When antibodies or other substances in serum cross-react with the test reagents,a false positive result can occur.

Correct AnswerverifiedShow Answer

You are the supervisor of a new reference microbiology laboratory,which will receive clinical specimens for identification of bacterial pathogens.When choosing the types of media to order for culture and isolation procedures in your laboratory,what question(s) do you need to answer?

Correct AnswerverifiedShow Answer

Which statement about clinical identification tests is true?

Correct AnswerverifiedShow Answer

The indirect ELISA test is commonly used to diagnose infections caused by all of the following except

Correct AnswerverifiedShow Answer

A positive serological test for tuberculosis indicates that the patient

Correct AnswerverifiedShow Answer

Microarrays,hybridization tests,and ribotyping are increasing in frequency in their use in clinical labs for diagnosis of infectious diseases and the microorganisms causing them.Why is this?

Correct AnswerverifiedShow Answer

Which of the following is not an agglutination test?

Correct AnswerverifiedShow Answer

Serological testing can be used to test each of the following except

Correct AnswerverifiedShow Answer

In precipitation tests,the antigen

Correct AnswerverifiedShow Answer

The direct ELISA test is used to diagnose all of the following except

Correct AnswerverifiedShow Answer

The tuberculin skin test is read

Correct AnswerverifiedShow Answer

Which of the following is not a genotypic method of identification?

Correct AnswerverifiedShow Answer

The polymerase chain reaction can be used for identification of:

Correct AnswerverifiedShow Answer

The tuberculin test is an example of an in vitro serological test.

Correct AnswerverifiedShow Answer

Correct Answer
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